Human microRNA sequencing and cytomegalovirus infection risk after kidney transplantation.

Cytomegalovirus (CMV)-seropositive kidney transplant recipients (KTRs) with detectable CMV-specific cell-mediated immunity according to the QuantiFERON-CMV assay (QTF-CMV) are expected to have adequate immune protection. Nevertheless, a proportion of patients still develop CMV infection. Human microRNAs (hsa-miRNAs) are promising biomarkers owing to their high stability and easy detection. We performed whole blood miRNA sequencing in samples coincident with the first reactive QTF-CMV after transplantation or cessation of antiviral prophylaxis to investigate hsa-miRNAs differentially expressed according to the occurrence of CMV infection. One-year incidence of CMV viremia was 55.0% (median interval from miRNA sequencing sampling of 29 days). After qPCR validation, we found that hsa-miR-125a-5p was downregulated in KTRs developing CMV viremia within the next 90 days (ΔCt: 7.9 ± 0.9 versus 7.3 ± 1.0; P = .011). This difference was more evident among KTRs preemptively managed (8.2 ± 0.9 versus 6.9 ± 0.8; P < .001), with an area under the receiver operating characteristic curve of 0.865. Functional enrichment analysis identified hsa-miR-125a-5p targets involved in cell cycle regulation and apoptosis, including the BAK1 gene, which was significantly downregulated in KTRs developing CMV viremia. In conclusion, hsa-miR-125a-5p may serve as biomarker to identify CMV-seropositive KTRs at risk of CMV reactivation despite detectable CMV-CMI.

Dizziness Handicap Inventory and Visual Vertigo Analog Scale in Vestibular Dysfunction

Abstract Introduction Dizziness is one of the most common symptoms among the population, producing numerous consequences for individual's quality of life. There are some questionnaires that can trace the patient's profile and quality of life impairment from dizziness, including the Dizziness Handicap Inventory (DHI) and the Visual Vertigo Analogue Scale (VVAS). Objective This study aims to correlate the results of the DHI and VVAS in patients with vestibular dysfunction. Methods This is a retrospective study of medical records of patients treated in a medical school between 2006 and 2012. Results of the DHI and EVA were collected and subjected to statistical analysis using Pearson's correlation test with p < 0.001. The significance level adopted for the statistical tests was p 0.05. Results A total of 91 records were included in this study, 72 (79.1%) from female and 19 (20.9%) from male patients, aged 23 to 86 years, with a mean age of 52.5 years. The mean score on the DHI total was 43.9 and 5.2 points for the EVA. The result of Pearson's correlation test was 0.54. Conclusion Self-perceived dizziness measured with the Dizziness Handicap Inventory has a regular and positive correlation with the Visual Vertigo Analog Scale in patients with vestibular dysfunction. The clinical trial is registered under number UTN U1111- 1170-5065.

Dizziness Handicap Inventory and Visual Vertigo Analog Scale in Vestibular Dysfunction.

INTRODUCTION: Dizziness is one of the most common symptoms among the population, producing numerous consequences for individual's quality of life. There are some questionnaires that can trace the patient's profile and quality of life impairment from dizziness, including the Dizziness Handicap Inventory (DHI) and the Visual Vertigo Analogue Scale (VVAS). OBJECTIVE: This study aims to correlate the results of the DHI and VVAS in patients with vestibular dysfunction. METHODS: This is a retrospective study of medical records of patients treated in a medical school between 2006 and 2012. Results of the DHI and EVA were collected and subjected to statistical analysis using Pearson's correlation test with p < 0.001. The significance level adopted for the statistical tests was p ≤ 0.05. RESULTS: A total of 91 records were included in this study, 72 (79.1%) from female and 19 (20.9%) from male patients, aged 23 to 86 years, with a mean age of 52.5 years. The mean score on the DHI total was 43.9 and 5.2 points for the EVA. The result of Pearson's correlation test was 0.54. CONCLUSION: Self-perceived dizziness measured with the Dizziness Handicap Inventory has a regular and positive correlation with the Visual Vertigo Analog Scale in patients with vestibular dysfunction. The clinical trial is registered under number UTN U1111-1170-5065.

Vestibular rehabilitation with visual stimuli in peripheral vestibular disorders / Reabilitação vestibular com estímulos visuais nas vestibulopatias periféricas

ABSTRACT INTRODUCTION: Visual stimuli can induce vestibular adaptation and recovery of body balance. OBJECTIVE: To verify the effect of visual stimuli by digital images on vestibular and body balance rehabilitation of peripheral vestibular disorders. METHODS: Clinical, randomized, prospective study. Forty patients aged between 23 and 63 years with chronic peripheral vestibular disorders underwent 12 sessions of rehabilitation with visual stimuli using digital video disk (DVD) (experimental group) or Cawthorne-Cooksey exercises (control group). The Dizziness Handicap Inventory (DHI), dizziness analog scale, and the sensitized Romberg static balance and one-leg stance tests were applied before and after the intervention. RESULTS: Before and after the intervention, there was no difference between the experimental and control groups (p > 0.005) regarding the findings of DHI, dizziness analog scale, and static balance tests. After the intervention, the experimental and control groups showed lower values (p < 0.05) in the DHI and the dizziness analog scale, and higher values (p < 0.05) in the static balance tests in some of the assessed conditions. CONCLUSION: The inclusion of visual stimuli by digital images on vestibular and body balance rehabilitation is effective in reducing dizziness and improving quality of life and postural control in individuals with peripheral vestibular disorders.

RESUMO INTRODUÇÃO: Estímulos visuais podem induzir a adaptação vestibular e recuperar o equilíbrio corporal. OBJETIVO: Verificar o efeito de estímulos visuais por imagens digitais na reabilitação do equilíbrio corporal de vestibulopatias periféricas. MÉTODO: Estudo clínico, randomizado, prospectivo. Quarenta pacientes com vestibulopatia periférica crônica e idade entre 23 e 63 anos foram submetidos à 12 sessões de reabilitação com estímulos visuais em DVD (grupo experimental) ou aos exercícios de Cawthorne-Cooksey (grupo controle). Dizziness Handicap Inventory (DHI), escala visual analógica de tontura e testes de equilíbrio estático de Romberg sensibilizado e de apoio unipodal foram aplicados antes e após a intervenção. RESULTADOS: Antes e após a intervenção, não houve diferença entre os grupos experimental e controle (p > 0,005) no DHI, escala visual analógica e testes de equilíbrio estático. Após a intervenção, o grupo experimental e o controle apresentaram valores menores (p < 0,05) do DHI e da escala visual analógica de tontura e valores maiores (p < 0,05) nos testes de equilíbrio estático em algumas condições avaliadas. CONCLUSÃO: A inclusão de estímulos visuais por imagens digitais na reabilitação do equilíbrio corporal é eficaz na redução da tontura, na melhora da qualidade de vida e do controle postural de vestibulopatias periféricas.

Vestibular rehabilitation with visual stimuli in peripheral vestibular disorders.

INTRODUCTION: Visual stimuli can induce vestibular adaptation and recovery of body balance. OBJECTIVE: To verify the effect of visual stimuli by digital images on vestibular and body balance rehabilitation of peripheral vestibular disorders. METHODS: Clinical, randomized, prospective study. Forty patients aged between 23 and 63 years with chronic peripheral vestibular disorders underwent 12 sessions of rehabilitation with visual stimuli using digital video disk (DVD) (experimental group) or Cawthorne-Cooksey exercises (control group). The Dizziness Handicap Inventory (DHI), dizziness analog scale, and the sensitized Romberg static balance and one-leg stance tests were applied before and after the intervention. RESULTS: Before and after the intervention, there was no difference between the experimental and control groups (p>0.005) regarding the findings of DHI, dizziness analog scale, and static balance tests. After the intervention, the experimental and control groups showed lower values (p<0.05) in the DHI and the dizziness analog scale, and higher values (p<0.05) in the static balance tests in some of the assessed conditions. CONCLUSION: The inclusion of visual stimuli by digital images on vestibular and body balance rehabilitation is effective in reducing dizziness and improving quality of life and postural control in individuals with peripheral vestibular disorders.

Influência do nistagmo espontâneo de olhos fechados na vectonistagmografia computadorizada em pacientes com vestibulopatias periféricas crônicas / Influence of spontaneous nystagmus with eyes closed on computerized vestibular exam of patients with chronic peripheral

Introdução: O nistagmo espontâneo de olhos fechados (NEOF) pode modificar os resultados obtidos durante algumas provas do exame vestibular, levando a conclusões errôneas. Objetivo: Caracterizar os pacientes e o tipo de influência do NEOF nas provas da vectonistagmografia digital. Método: Estudo retrospectivo baseado no levantamento de prontuários de pacientes com presença de NEOF à vectonistagmografia digital, atendidos no Ambulatório de Equilibriometria da UNIFESP-EPM, nos anos de 2000 a 2007. Foram realizadas comparações entre gêneros, idades, direção, velocidade angular do NEOF, conclusão do exame vestibular e sua influência nos resultados da prova calórica. Resultados: Encontramos 73,7% população do sexo feminino; média etária de 55,08 anos; prevalência do NEOF horizontais e velocidade angular menor do que 7o/s em 86,7%. 59% da amostra apresentaram algum tipo de influência causado pelo NEOF na prova calórica como: inversão, hiporreflexia, hiper-reflexia, preponderância direcional do nistagmo (PDN) e preponderância labiríntica (PL) alteradas. As conclusões mais prevalentes foram Exame Vestibular Normal (EVN) e síndrome vestibular periférica irritativa bilateral (SVPIB). 38,7% realizaram prova calórica gelada e desses casos foi possível chegar a uma conclusão em 79%. Conclusão: O NEOF mais comum foi do tipo horizontal, com VACL menor do que 7o/s, influenciando na maioria dos exames e somente nos resultados da prova calórica a ar, com inversão do nistagmo pós-calórico, hiper-reflexia e hiporreflexia, PDN e PL alteradas; as conclusões mais prevalentes foram EVN e SVPIB; e a prova calórica gelada retirou a influência do NEOF na maioria dos indivíduos, possibilitando chegar a uma conclusão final.

Introduction: The spontaneous nystagmus with eyes closed (NEOF) can modify the results obtained during some evidence of vestibular, leading to erroneous conclusions. Objective: To characterize the patients and the type of influence on the evidence of NEOF vectonystagmography digital. Method: Retrospective study based on survey charts of patients with the presence of NEOF vectonystagmography digital, in the Outpatient Equilibriometria UNIFESP-EPM, in the years 2000 to 2007. Comparisons were made between genders, ages, direction, angular velocity of NEOF, completion of entrance examination and its influence on the results of caloric testing. Results: We found 73.7% of the population was female, mean age of 55.08 years; NEOF prevalence of horizontal and angular velocity smaller than 7o/s in 86.7%. 59% had some kind of influence caused by the caloric test NEOF as inversion, hyporeflexia, hyperreflexia, nystagmus directional preponderance (NDP) and labyrinthine preponderance (LP) changed. The most prevalent findings were normal vestibular tests (EVN) and bilateral irritative peripheral vestibular syndrome (BIPVS). 38.7% caloric ice and realized these cases it was possible to reach a conclusion in 79%. Conclusion: The NEOF most common was the horizontal type, with VACL less than 7o/s, influencing the majority of examinations and only the results of caloric testing the air, with reversal of post-caloric nystagmus, hyperreflexia and hyporeflexia, NDP and PL altered; the findings were more prevalent and BIPVS EVN, and the caloric test the influence of ice withdrew NEOF in most individuals, enabling to reach a final conclusion.

Achados à prova calórica e canal semicircular acometido na vertigem posicional paroxística benigna / Caloric test results and damaged semicircular canal in benign positional paroxysmal vertigo

OBJETIVO: Caracterizar os achados da prova calórica em pacientes com vertigem posicional paroxística benigna, segundo o canal semicircular comprometido. MÉTODOS: Foram analisados 1033 prontuários de pacientes submetidos à pesquisa de nistagmo posicional e de posicionamento e à eletronistagmografia. Os achados da prova calórica, de acordo com os canais semicirculares acometidos, foram submetidos à análise estatística. RESULTADOS: No comprometimento de canal posterior, houve prevalência de normorreflexia (p<0,0001); hiporreflexia prevaleceu sobre hiper-reflexia (p<0,0001) e preponderância direcional (p<0,0001), e hiper-reflexia prevaleceu sobre preponderância direcional (p<0,0001). No comprometimento de canal lateral, normorreflexia prevaleceu sobre hiporreflexia (p<0,0001) e hiper-reflexia (p<0,0001); hiporreflexia tendeu a prevalecer sobre hiper-reflexia (p=0,0771), e preponderância direcional não ocorreu. No comprometimento de canal anterior, normorreflexia prevaleceu sobre hiporreflexia (p<0,0001); hiper-reflexia e preponderância direcional não ocorreram. CONCLUSÃO: Na prova calórica de pacientes com vertigem posicional paroxística benigna, normorreflexia, hiporreflexia, hiper-reflexia ou preponderância direcional do nistagmo pós-calórico ocorrem em ordem decrescente de prevalência, quando há comprometimento do canal posterior; normorreflexia prevalece sobre hiporreflexia ou hiper-reflexia e hiporreflexia tende a prevalecer sobre hiper-reflexia, no comprometimento do canal lateral; normorreflexia prevalece sobre hiporreflexia, no comprometimento do canal anterior.

PURPOSE: To characterize caloric test results in benign paroxysmal positional vertigo patients, according to the damaged semicircular canal. METHODS: The data of 1033 patients submitted to Dix-Hallpike testing, positional nystagmus and electronystagmography were analyzed. Caloric test results were compared to the damaged semicircular canals and were submitted to statistical analysis. RESULTS: In cases with posterior canal damage, there was prevalence of normal responses compared to abnormal results (p<0.0001); hypo activity was more prevalent than hyperactivity (p<0.0001) and directional preponderance (p<0.0001), and hyperactivity more prevalent than directional preponderance (p<0.0001). In cases with lateral canal damage, normal responses were more prevalent than hypo activity (p<0.0001) and hyperactivity (p<0.0001); there was a tendency of prevalence of hypo activity over hyperactivity (p=0.0771), and directional preponderance was not observed. In cases with anterior canal damage, normal responses were more prevalent than hypo activity (p<0.0001); hyperactivity and directional preponderance were not observed. CONCLUSION: In the caloric test of benign positional paroxysmal vertigo patients, normal responses, hypo activity, hyperactivity or directional preponderance of post-caloric nystagmus occur in decreasing order of prevalence when the posterior canal is damaged; normal responses are more prevalent than hypoactive or hyperactive caloric responses, and there is a tendency of prevalence of hypoactive over hyperactive caloric responses when the lateral canal is damaged; normal responses are more prevalent than hypoactive caloric responses when the anterior canal is damaged.

Reabilitação vestibular personalizada: levantamento de prontuários dos pacientes atendidos no ambulatório de otoneurologia da I. S. C. M. S. P / Personalized vestibular rehabilitation: medical chart survey with patients seen at the ambulatory of otoneurology of I. S. C. M. S. P

O OBJETIVO: o objetivo deste trabalho foi verificar a eficácia da reabilitação vestibular personalizada (RVP) em diferentes quadros otoneurológicos. FORMA DE ESTUDO: clínico retrospectivo. MATERIAL E MÉTODO: Foi realizado um estudo retrospectivo que constou de uma descrição do programa de reabilitação vestibular de 37 pacientes, com idade entre 21 a 87 anos, vinte e seis do sexo feminino, onze do sexo masculino, com quadros otoneurológicos variados, atendidos no Ambulatório de Otoneurologia do Departamento de Otorrinolaringologia da Irmandade da Santa Casa de Misericórdia de São Paulo, no período de 2002 a 2003. Estes foram submetidos à avaliação otoneurológica e depois de realizado o diagnóstico pelo otorrinolaringologista, encaminhados para a reabilitação vestibular, realizada semanalmente no ambulatório, e em casa diariamente. Cada paciente seguiu programas específicos de exercícios, considerando-se os achados ao exame vestibular, o quadro clínico e, principalmente, os sintomas apresentados. Os exercícios foram baseados em diferentes protocolos descritos na literatura. Foi realizada uma análise individual da evolução clínica e em conjunto, a fim de verificar a eficácia da RVP em diferentes quadros clínicos. CONCLUSÃO: Pudemos concluir que o programa de RVP mostrou-se um recurso terapêutico efetivo na diminuição e extinção dos sintomas e conseqüente melhora na qualidade de vida de pacientes portadores de diferentes quadros clínicos.

Personalized vestibular rehabilitation: medical chart survey with patients seen at the ambulatory of otoneurology of I.S.C.M.S.P.

UNLABELLED: The objective of this research study was to verify the efficiency of the personalized vestibular rehabilitation (PVR) in different otoneurologic clinical diseases, as well as set the best protocol option in each case. STUDY DESIGN: Clinical retrospective. MATERIAL AND METHOD: A retrospective study was conducted based on the description of the vestibular rehabilitation program of 37 patients aged 21 to 87 years, twenty-six females and eleven males, with different clinical diseases seen in the Otoneurologic Ambulatory of Otolaryngology, department of Irmandade da Santa Casa de Misericórdia de Sao Paulo, from 2002 to 2003. Those patients went through otoneurologic evaluation and after diagnosis they were referred to vestibular rehabilitation. Each patient followed a specific program based on diagnosis, clinical disease and symptoms. We performed an individual analysis of the evaluation of each patient and group analysis in order to verify the efficiency of the PVR. CONCLUSION: It was possible to conclude that the PVR program is an effective resource in the treatment of otoneurologic symptoms of patients, consequently improving their quality of life.